This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for RFC1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFC1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFC1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:39,135,136–39,135,640 | 230.9 kb | Distal (>10kb) Multiome | 399 | |
| chr4:39,181,955–39,183,542 | 184.0 kb | Distal (>10kb) Multiome | 644 | |
| chr4:39,365,894–39,366,933 | 7 bp | At TSS Multiome | 1045 | |
| chr4:39,446,525–39,447,613 | 80.8 kb | Distal (>10kb) Multiome | 443 | |
| chr4:39,458,264–39,459,604 | 92.6 kb | Distal (>10kb) Multiome | 1000 | |
| chr4:39,526,691–39,528,369 | 161.3 kb | Distal (>10kb) Multiome | 1061 | |
| chr4:39,558,019–39,558,995 | 192.1 kb | Distal (>10kb) Multiome | 500 | |
| chr4:39,635,065–39,635,553 | 268.9 kb | Distal (>10kb) Multiome | 157 | |
| chr4:39,637,938–39,639,954 | 272.7 kb | Distal (>10kb) Multiome | 976 |
Genomic view of the RFC1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.