Enables several functions, including identical protein binding activity; transcription coactivator activity; and ubiquitin binding activity. Involved in several processes, including positive regulation of canonical NF-kappaB signal transduction; protein linear polyubiquitination; and regulation of DNA-binding transcription factor activity. Part of LUBAC complex. Implicated in glycogen storage disease. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for RBCK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RBCK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RBCK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:266,689–267,291 | 141.2 kb | Distal (>10kb) Multiome | 931 | |
| chr20:290,009–290,864 | 117.8 kb | Distal (>10kb) Multiome | 706 | |
| chr20:297,159–298,781 | 110.8 kb | Distal (>10kb) Multiome | 1084 | |
| chr20:300,984–301,434 | 107.0 kb | Distal (>10kb) Multiome | 252 | |
| chr20:309,597–310,812 | 97.8 kb | Distal (>10kb) Multiome | 589 | |
| chr20:323,634–328,069 | 81.0 kb | Distal (>10kb) Multiome | 1260 | |
| chr20:330,299–330,873 | 77.6 kb | Distal (>10kb) Multiome | 376 | |
| chr20:346,592–348,332 | 60.4 kb | Distal (>10kb) Multiome | 1061 | |
| chr20:354,335–354,944 | 53.6 kb | Distal (>10kb) Multiome | 631 | |
| chr20:380,569–381,493 | 27.4 kb | Distal (>10kb) Multiome | 958 | |
| chr20:381,625–382,263 | 26.2 kb | Distal (>10kb) Multiome | 745 | |
| chr20:407,136–409,109 | 274 bp | At TSS Multiome | 1064 | |
| chr20:461,861–463,102 | 54.2 kb | Distal (>10kb) Multiome | 871 | |
| chr20:543,069–544,398 | 135.6 kb | Distal (>10kb) Multiome | 965 | |
| chr20:555,914–556,523 | 147.9 kb | Distal (>10kb) Multiome | 249 | |
| chr20:574,398–574,909 | 166.4 kb | Distal (>10kb) Multiome | 119 | |
| chr20:609,984–610,596 | 202.0 kb | Distal (>10kb) Multiome | 205 | |
| chr20:652,813–653,697 | 245.1 kb | Distal (>10kb) Multiome | 897 | |
| chr20:675,301–676,096 | 267.4 kb | Distal (>10kb) Multiome | 782 |
Genomic view of the RBCK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.