This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]
Transcription factors with Perturb-seq knockdown data for RASSF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RASSF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RASSF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:50,088,748–50,089,596 | 248.5 kb | Distal (>10kb) Multiome | 677 | |
| chr3:50,121,279–50,122,037 | 215.7 kb | Distal (>10kb) Multiome | 364 | |
| chr3:50,138,509–50,140,035 | 198.1 kb | Distal (>10kb) Multiome | 198 | |
| chr3:50,154,437–50,155,741 | 182.4 kb | Distal (>10kb) Multiome | 450 | |
| chr3:50,204,913–50,205,545 | 132.2 kb | Distal (>10kb) Multiome | 367 | |
| chr3:50,205,645–50,206,357 | 131.3 kb | Distal (>10kb) Multiome | 347 | |
| chr3:50,226,120–50,228,328 | 111.3 kb | Distal (>10kb) Multiome | 915 | |
| chr3:50,235,551–50,236,824 | 101.4 kb | Distal (>10kb) Multiome | 632 | |
| chr3:50,240,985–50,241,805 | 96.2 kb | Distal (>10kb) Multiome | 137 | |
| chr3:50,245,721–50,247,161 | 91.2 kb | Distal (>10kb) Multiome | 671 | |
| chr3:50,259,568–50,260,922 | 77.2 kb | Distal (>10kb) Multiome | 869 | |
| chr3:50,264,309–50,265,446 | 72.6 kb | Distal (>10kb) Multiome | 568 | |
| chr3:50,275,163–50,276,639 | 61.0 kb | Distal (>10kb) Multiome | 151 | |
| chr3:50,291,628–50,292,796 | 44.9 kb | Distal (>10kb) Multiome | 855 | |
| chr3:50,298,495–50,300,110 | 38.0 kb | Distal (>10kb) Multiome | 872 | |
| chr3:50,303,363–50,303,840 | 33.8 kb | Distal (>10kb) Multiome | 484 | |
| chr3:50,320,603–50,322,739 | 15.9 kb | Distal (>10kb) Multiome | 816 | |
| chr3:50,324,456–50,325,020 | 12.7 kb | Distal (>10kb) Multiome | 392 | |
| chr3:50,327,933–50,328,581 | 9.2 kb | Proximal (<10kb) Multiome | 697 | |
| chr3:50,336,856–50,338,227 | 93 bp | At TSS Multiome | 952 | |
| chr3:50,340,624–50,341,322 | 3.5 kb | Proximal (<10kb) Multiome | 345 | |
| chr3:50,345,443–50,346,042 | 8.3 kb | Proximal (<10kb) Multiome | 209 | |
| chr3:50,350,496–50,351,298 | 13.5 kb | Distal (>10kb) Multiome | 902 | |
| chr3:50,358,889–50,360,012 | 22.1 kb | Distal (>10kb) Multiome | 796 | |
| chr3:50,364,552–50,365,867 | 27.8 kb | Distal (>10kb) Multiome | 331 | |
| chr3:50,435,932–50,436,533 | 98.7 kb | Distal (>10kb) Multiome | 248 | |
| chr3:50,502,505–50,504,160 | 166.2 kb | Distal (>10kb) Multiome | 312 | |
| chr3:50,567,311–50,568,123 | 230.3 kb | Distal (>10kb) Multiome HiCAR | 376 | |
| chr3:50,568,824–50,569,934 | 231.8 kb | Distal (>10kb) Multiome HiCAR | 901 | |
| chr3:50,611,315–50,612,530 | 274.5 kb | Distal (>10kb) Multiome HiCAR | 904 | |
| chr3:50,615,997–50,617,867 | 279.7 kb | Distal (>10kb) Multiome HiCAR | 754 | |
| chr3:51,982,506–51,983,633 | 1645.8 kb | Distal (>10kb) Multiome HiCAR | 933 |
Genomic view of the RASSF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.