RAD50
RAD50 double strand break repair protein | RAD50-2, hRad50

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Member of: DE-3
Biological processes 61 terms
3'-5' exonuclease activity (GO:0008408)ATP hydrolysis activity (GO:0016887)ATP hydrolysis activity (GO:0016887)DNA binding (GO:0003677)DNA damage response (GO:0006974)DNA double-strand break processing (GO:0000729)DNA double-strand break processing (GO:0000729)DNA helicase activity (GO:0003678)DNA recombination (GO:0006310)DNA repair (GO:0006281)DNA repair (GO:0006281)DNA strand resection involved in replication fork processing (GO:0110025)DNA strand resection involved in replication fork processing (GO:0110025)G-quadruplex DNA binding (GO:0051880)Mre11 complex (GO:0030870)Mre11 complex (GO:0030870)Mre11 complex (GO:0030870)Mre11 complex (GO:0030870)R-loop processing (GO:0062176)chromosomal region (GO:0098687)chromosome (GO:0005694)chromosome organization (GO:0051276)chromosome organization involved in meiotic cell cycle (GO:0070192)chromosome, telomeric region (GO:0000781)chromosome, telomeric region (GO:0000781)chromosome, telomeric region (GO:0000781)chromosome, telomeric region (GO:0000781)condensed nuclear chromosome (GO:0000794)double-strand break repair (GO:0006302)double-strand break repair (GO:0006302)double-strand break repair (GO:0006302)double-strand break repair (GO:0006302)double-strand break repair via homologous recombination (GO:0000724)double-stranded telomeric DNA binding (GO:0003691)homologous recombination (GO:0035825)identical protein binding (GO:0042802)membrane (GO:0016020)mitotic G2/M transition checkpoint (GO:0044818)negative regulation of telomere capping (GO:1904354)nuclear body (GO:0016604)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of double-strand break repair (GO:2000781)positive regulation of telomere maintenance (GO:0032206)protein binding (GO:0005515)protein serine/threonine kinase activator activity (GO:0043539)protein-macromolecule adaptor activity (GO:0030674)reciprocal meiotic recombination (GO:0007131)regulation of mitotic recombination (GO:0000019)single-stranded DNA endonuclease activity (GO:0000014)single-stranded telomeric DNA binding (GO:0043047)site of double-strand break (GO:0035861)site of double-strand break (GO:0035861)telomere maintenance (GO:0000723)telomere maintenance (GO:0000723)telomere maintenance via recombination (GO:0000722)telomere maintenance via telomerase (GO:0007004)telomere maintenance via telomerase (GO:0007004)telomeric 3' overhang formation (GO:0031860)
Expression (TPM)
RAD50 — as a Regulated Gene

TFs regulating RAD50 0 TFs

Transcription factors with Perturb-seq knockdown data for RAD50. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAD50 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to RAD50

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAD50, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr5:132,256,858–132,258,371 299.4 kb Distal (>10kb) Multiome 338
chr5:132,293,061–132,295,661 262.8 kb Distal (>10kb) Multiome 1053
chr5:132,369,150–132,370,578 187.2 kb Distal (>10kb) Multiome 819
chr5:132,410,330–132,411,433 146.0 kb Distal (>10kb) Multiome 815
chr5:132,419,541–132,419,986 137.1 kb Distal (>10kb) Multiome 481
chr5:132,426,660–132,427,851 129.9 kb Distal (>10kb) Multiome 540
chr5:132,489,909–132,492,004 66.1 kb Distal (>10kb) Multiome 899
chr5:132,495,550–132,497,614 60.5 kb Distal (>10kb) Multiome 946
chr5:132,526,534–132,527,026 30.1 kb Distal (>10kb) Multiome 329
chr5:132,556,575–132,557,723 48 bp At TSS Multiome 1071
chr5:132,655,819–132,656,736 99.3 kb Distal (>10kb) Multiome 235
chr5:132,736,904–132,738,172 180.6 kb Distal (>10kb) Multiome 706
chr5:132,746,941–132,748,230 190.8 kb Distal (>10kb) Multiome 369
chr5:132,776,389–132,777,915 220.4 kb Distal (>10kb) Multiome 587
chr5:132,813,194–132,815,033 257.6 kb Distal (>10kb) Multiome 418
chr5:132,822,729–132,823,756 266.3 kb Distal (>10kb) Multiome 258
chr5:132,825,176–132,826,484 268.8 kb Distal (>10kb) Multiome 208
chr5:132,829,647–132,831,156 273.4 kb Distal (>10kb) Multiome 877

Genome Browser

Genomic view of the RAD50 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr5:132,246,858 – 132,841,156
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq
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