The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for RAD17. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAD17 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAD17, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:69,093,377–69,094,772 | 275.9 kb | Distal (>10kb) Multiome | 952 | |
| chr5:69,166,575–69,167,819 | 202.8 kb | Distal (>10kb) Multiome | 840 | |
| chr5:69,188,979–69,190,115 | 180.2 kb | Distal (>10kb) Multiome | 898 | |
| chr5:69,217,542–69,218,587 | 151.8 kb | Distal (>10kb) Multiome | 898 | |
| chr5:69,234,302–69,235,305 | 135.0 kb | Distal (>10kb) Multiome | 732 | |
| chr5:69,300,544–69,301,286 | 69.0 kb | Distal (>10kb) Multiome | 217 | |
| chr5:69,368,998–69,370,235 | 140 bp | At TSS Multiome | 956 | |
| chr5:69,414,852–69,415,897 | 45.3 kb | Distal (>10kb) Multiome | 714 | |
| chr5:69,492,169–69,494,057 | 122.9 kb | Distal (>10kb) Multiome | 821 | |
| chr5:69,499,604–69,500,518 | 130.3 kb | Distal (>10kb) Multiome | 300 |
Genomic view of the RAD17 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.