This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PYCARD. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PYCARD upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PYCARD, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:30,901,969–30,902,794 | 300.4 kb | Distal (>10kb) Multiome | 579 | |
| chr16:30,921,611–30,925,097 | 279.4 kb | Distal (>10kb) Multiome | 1132 | |
| chr16:30,927,705–30,928,596 | 274.6 kb | Distal (>10kb) Multiome | 274 | |
| chr16:30,941,803–30,942,254 | 260.7 kb | Distal (>10kb) Multiome | 507 | |
| chr16:30,947,471–30,950,635 | 252.5 kb | Distal (>10kb) Multiome | 1066 | |
| chr16:30,953,370–30,954,135 | 248.9 kb | Distal (>10kb) Multiome | 499 | |
| chr16:30,956,960–30,958,498 | 245.1 kb | Distal (>10kb) Multiome | 1022 | |
| chr16:30,984,837–30,985,709 | 217.6 kb | Distal (>10kb) Multiome | 787 | |
| chr16:30,996,877–30,998,001 | 205.4 kb | Distal (>10kb) Multiome | 759 | |
| chr16:31,010,379–31,011,032 | 192.1 kb | Distal (>10kb) Multiome | 117 | |
| chr16:31,032,789–31,034,448 | 169.3 kb | Distal (>10kb) Multiome | 1047 | |
| chr16:31,041,996–31,042,665 | 160.4 kb | Distal (>10kb) Multiome | 213 | |
| chr16:31,072,597–31,074,957 | 129.3 kb | Distal (>10kb) Multiome | 1167 | |
| chr16:31,094,132–31,095,139 | 107.9 kb | Distal (>10kb) Multiome | 933 | |
| chr16:31,108,209–31,108,826 | 94.4 kb | Distal (>10kb) Multiome | 733 | |
| chr16:31,117,128–31,118,265 | 85.0 kb | Distal (>10kb) Multiome | 929 | |
| chr16:31,134,682–31,136,040 | 67.0 kb | Distal (>10kb) Multiome | 450 | |
| chr16:31,138,695–31,139,457 | 63.7 kb | Distal (>10kb) Multiome | 413 | |
| chr16:31,141,455–31,143,270 | 60.4 kb | Distal (>10kb) Multiome | 856 | |
| chr16:31,148,229–31,148,775 | 54.3 kb | Distal (>10kb) Multiome | 209 | |
| chr16:31,179,340–31,181,495 | 22.7 kb | Distal (>10kb) Multiome | 1179 | |
| chr16:31,201,568–31,203,049 | 557 bp | At TSS Multiome | 507 | |
| chr16:31,214,606–31,216,091 | 12.5 kb | Distal (>10kb) Multiome | 469 | |
| chr16:31,406,131–31,406,947 | 203.7 kb | Distal (>10kb) Multiome HiCAR | 214 | |
| chr16:31,442,550–31,443,636 | 240.3 kb | Distal (>10kb) Multiome HiCAR | 838 | |
| chr16:31,457,952–31,460,456 | 255.3 kb | Distal (>10kb) Multiome | 1128 | |
| chr16:31,471,780–31,473,168 | 270.1 kb | Distal (>10kb) Multiome | 425 | |
| chr16:31,476,391–31,478,207 | 275.1 kb | Distal (>10kb) Multiome | 750 | |
| chr16:31,487,298–31,488,862 | 285.1 kb | Distal (>10kb) Multiome | 323 |
Genomic view of the PYCARD locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.