Transcription factors with Perturb-seq knockdown data for PRKCZ-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKCZ-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKCZ-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:2,044,569–2,045,304 | 4.8 kb | Proximal (<10kb) | 403 | |
| chr1:2,046,521–2,046,771 | 3.3 kb | Proximal (<10kb) | 352 | |
| chr1:2,047,086–2,047,770 | 2.3 kb | Proximal (<10kb) | 290 | |
| chr1:2,049,277–2,050,992 | at TSS | At TSS | 732 |
Genomic view of the PRKCZ-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.