This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]
Modules significantly affected by knockdown. ↑ Up = module upregulated upon KD; ↓ Down = module downregulated upon KD.
| Module | Dir | NES | #gRNA | padj | Bind | OR | padj (bind) |
|---|
| Submodule | Module | Dir | NES | #gRNA | Bind | OR | padj (bind) |
|---|
Genes likely regulated by PRDM14 through linked binding evidence in open chromatin. The chart ranks TF-linked genes by their mean Perturb-seq response to PRDM14 knockdown, with negative coefficients indicating downregulation and positive coefficients indicating upregulation upon knockdown.
Open chromatin elements (ATAC-seq) where PRDM14 has ChIP-seq or motif footprint binding evidence and which are linked to at least one target gene region.
| Element | Size | Linked genes |
|---|
Transcription factors with Perturb-seq knockdown data for PRDM14. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRDM14 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRDM14, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:69,831,934–69,835,337 | 238.7 kb | Distal (>10kb) Multiome | 736 | |
| chr8:69,977,364–69,977,835 | 93.7 kb | Distal (>10kb) Multiome HiCAR | 156 | |
| chr8:69,995,289–69,995,821 | 75.6 kb | Distal (>10kb) Multiome | 42 | |
| chr8:70,034,558–70,035,468 | 36.2 kb | Distal (>10kb) Multiome | 244 | |
| chr8:70,060,654–70,062,013 | 9.8 kb | Proximal (<10kb) Multiome | 276 | |
| chr8:70,065,127–70,065,558 | 5.7 kb | Proximal (<10kb) | 45 | |
| chr8:70,068,781–70,072,924 | 119 bp | At TSS Multiome | 400 | |
| chr8:70,217,022–70,217,589 | 146.1 kb | Distal (>10kb) Multiome | 237 |
Genomic view of the PRDM14 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.