This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
Transcription factors with Perturb-seq knockdown data for PRCP. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRCP upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRCP, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:82,695,267–82,696,305 | 204.6 kb | Distal (>10kb) Multiome | 385 | |
| chr11:82,732,363–82,734,437 | 166.6 kb | Distal (>10kb) Multiome HiCAR | 442 | |
| chr11:82,750,605–82,751,145 | 149.5 kb | Distal (>10kb) Multiome | 218 | |
| chr11:82,775,808–82,776,868 | 124.3 kb | Distal (>10kb) Multiome | 252 | |
| chr11:82,899,722–82,902,187 | 1.2 kb | Proximal (<10kb) Multiome | 1001 | |
| chr11:82,908,868–82,909,477 | 8.4 kb | Proximal (<10kb) | 35 | |
| chr11:82,970,431–82,971,628 | 70.4 kb | Distal (>10kb) Multiome | 188 | |
| chr11:83,034,012–83,034,897 | 134.1 kb | Distal (>10kb) Multiome | 343 | |
| chr11:83,071,319–83,072,558 | 171.5 kb | Distal (>10kb) Multiome HiCAR | 943 | |
| chr11:83,144,373–83,145,287 | 244.4 kb | Distal (>10kb) Multiome HiCAR | 76 | |
| chr11:83,156,155–83,158,430 | 256.4 kb | Distal (>10kb) Multiome HiCAR | 1001 | |
| chr11:83,193,357–83,194,770 | 293.3 kb | Distal (>10kb) Multiome HiCAR | 817 | |
| chr11:83,241,143–83,241,871 | 341.1 kb | Distal (>10kb) Multiome HiCAR | 285 | |
| chr11:83,724,266–83,725,551 | 824.6 kb | Distal (>10kb) Multiome HiCAR | 319 |
Genomic view of the PRCP locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.