Transcription factors with Perturb-seq knockdown data for PPP3CB-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPP3CB-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPP3CB-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:73,246,675–73,247,516 | 248.6 kb | Distal (>10kb) Multiome | 1023 | |
| chr10:73,252,167–73,253,067 | 243.1 kb | Distal (>10kb) Multiome | 929 | |
| chr10:73,358,383–73,358,977 | 137.0 kb | Distal (>10kb) Multiome | 468 | |
| chr10:73,413,302–73,414,453 | 81.7 kb | Distal (>10kb) Multiome | 938 | |
| chr10:73,495,235–73,496,398 | 166 bp | At TSS Multiome | 908 | |
| chr10:73,591,236–73,591,781 | 95.7 kb | Distal (>10kb) Multiome | 359 | |
| chr10:73,591,961–73,592,227 | 96.3 kb | Distal (>10kb) Multiome | 458 | |
| chr10:73,625,111–73,626,369 | 130.2 kb | Distal (>10kb) Multiome | 810 | |
| chr10:73,647,157–73,648,042 | 151.8 kb | Distal (>10kb) Multiome HiCAR | 238 | |
| chr10:73,730,165–73,730,854 | 234.8 kb | Distal (>10kb) Multiome | 823 | |
| chr10:73,741,689–73,742,673 | 246.4 kb | Distal (>10kb) Multiome | 190 | |
| chr10:73,743,801–73,744,894 | 248.6 kb | Distal (>10kb) Multiome | 962 | |
| chr10:73,771,782–73,773,237 | 277.0 kb | Distal (>10kb) Multiome | 918 | |
| chr10:73,781,688–73,782,352 | 286.3 kb | Distal (>10kb) Multiome | 721 | |
| chr10:73,785,180–73,785,869 | 289.7 kb | Distal (>10kb) Multiome | 745 |
Genomic view of the PPP3CB-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.