Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for PPCS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPCS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPCS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:42,334,342–42,336,315 | 121.3 kb | Distal (>10kb) Multiome | 1056 | |
| chr1:42,380,246–42,381,273 | 75.8 kb | Distal (>10kb) Multiome | 485 | |
| chr1:42,455,770–42,457,060 | 202 bp | At TSS Multiome | 881 | |
| chr1:42,462,753–42,463,783 | 6.6 kb | Proximal (<10kb) Multiome | 581 | |
| chr1:42,657,991–42,659,026 | 201.8 kb | Distal (>10kb) Multiome | 952 | |
| chr1:42,681,723–42,683,347 | 225.8 kb | Distal (>10kb) Multiome | 924 | |
| chr1:42,739,796–42,740,734 | 283.8 kb | Distal (>10kb) Multiome | 365 |
Genomic view of the PPCS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.