Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for POU5F2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POU5F2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POU5F2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:93,569,362–93,571,779 | 170.7 kb | Distal (>10kb) Multiome | 360 | |
| chr5:93,578,889–93,582,135 | 161.5 kb | Distal (>10kb) Multiome | 840 | |
| chr5:93,596,297–93,597,643 | 144.4 kb | Distal (>10kb) Multiome | 351 | |
| chr5:93,620,145–93,621,850 | 120.3 kb | Distal (>10kb) Multiome | 782 | |
| chr5:93,741,247–93,741,767 | at TSS | At TSS | 96 |
Genomic view of the POU5F2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.