Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II; positive regulation of DNA-templated transcription; and positive regulation of gene expression. Predicted to act upstream of or within keratinocyte differentiation; nervous system development; and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. [provided by Alliance of Genome Resources, Apr 2025]
Transcription factors with Perturb-seq knockdown data for POU3F1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POU3F1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POU3F1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:37,752,766–37,753,277 | 293.7 kb | Distal (>10kb) Multiome | 223 | |
| chr1:37,793,768–37,794,528 | 252.8 kb | Distal (>10kb) Multiome | 567 | |
| chr1:37,807,583–37,809,057 | 238.6 kb | Distal (>10kb) Multiome | 755 | |
| chr1:37,859,081–37,860,381 | 187.0 kb | Distal (>10kb) Multiome | 939 | |
| chr1:37,931,446–37,932,215 | 114.9 kb | Distal (>10kb) Multiome | 559 | |
| chr1:37,946,605–37,947,220 | 99.7 kb | Distal (>10kb) Multiome | 610 | |
| chr1:37,989,644–37,990,549 | 56.6 kb | Distal (>10kb) Multiome | 1133 | |
| chr1:37,995,790–37,996,610 | 50.6 kb | Distal (>10kb) Multiome | 313 | |
| chr1:38,004,771–38,006,024 | 41.2 kb | Distal (>10kb) Multiome | 442 | |
| chr1:38,012,220–38,013,284 | 34.2 kb | Distal (>10kb) Multiome | 1074 | |
| chr1:38,045,344–38,048,035 | 206 bp | At TSS Multiome | 542 | |
| chr1:38,817,720–38,818,696 | 771.5 kb | Distal (>10kb) Multiome HiCAR | 375 | |
| chr1:38,859,087–38,860,584 | 813.0 kb | Distal (>10kb) Multiome HiCAR | 848 |
Genomic view of the POU3F1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.