POLR3F

RNA polymerase III subunit F | C34, RPC39, RPC6

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Biological processes 21 terms

4 iron, 4 sulfur cluster binding (GO:0051539)DNA-directed RNA polymerase activity (GO:0003899)RNA polymerase III complex (GO:0005666)RNA polymerase III complex (GO:0005666)RNA polymerase III complex (GO:0005666)RNA polymerase III complex (GO:0005666)cytoplasm (GO:0005737)cytosol (GO:0005829)double-stranded DNA binding (GO:0003690)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of innate immune response (GO:0045089)positive regulation of interferon-beta production (GO:0032728)protein binding (GO:0005515)regulation of transcription by RNA polymerase III (GO:0006359)termination of RNA polymerase III transcription (GO:0006386)transcription by RNA polymerase III (GO:0006383)transcription by RNA polymerase III (GO:0006383)transcription initiation at RNA polymerase III promoter (GO:0006384)

Expression (TPM)

TFs regulating POLR3F 0 TFs

Transcription factors with Perturb-seq knockdown data for POLR3F. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POLR3F upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to POLR3F

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POLR3F, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr20:18,287,674–18,288,982	179.1 kb	Distal (>10kb) Multiome	971
chr20:18,413,071–18,413,534	54.0 kb	Distal (>10kb) Multiome	167
chr20:18,466,666–18,467,699	283 bp	At TSS Multiome	930
chr20:18,496,758–18,497,430	29.8 kb	Distal (>10kb) Multiome	820
chr20:18,507,260–18,508,352	40.3 kb	Distal (>10kb) Multiome	959
chr20:18,566,948–18,567,920	100.0 kb	Distal (>10kb) Multiome	957
chr20:18,587,564–18,588,839	120.6 kb	Distal (>10kb) Multiome	882

Genome Browser

Genomic view of the POLR3F locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.