This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for POFUT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POFUT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POFUT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:31,951,523–31,952,606 | 255.8 kb | Distal (>10kb) Multiome | 879 | |
| chr20:31,967,880–31,968,893 | 239.4 kb | Distal (>10kb) Multiome | 1059 | |
| chr20:31,972,125–31,972,938 | 235.4 kb | Distal (>10kb) Multiome | 252 | |
| chr20:32,017,795–32,019,711 | 189.5 kb | Distal (>10kb) Multiome | 611 | |
| chr20:32,031,048–32,031,781 | 176.3 kb | Distal (>10kb) Multiome | 627 | |
| chr20:32,109,321–32,110,237 | 98.1 kb | Distal (>10kb) Multiome | 1073 | |
| chr20:32,189,679–32,190,832 | 17.5 kb | Distal (>10kb) Multiome | 290 | |
| chr20:32,207,261–32,208,118 | 69 bp | At TSS Multiome | 903 | |
| chr20:32,277,439–32,278,095 | 69.8 kb | Distal (>10kb) Multiome | 804 | |
| chr20:32,357,509–32,359,534 | 151.5 kb | Distal (>10kb) Multiome | 1138 | |
| chr20:32,456,960–32,457,933 | 249.6 kb | Distal (>10kb) Multiome | 407 | |
| chr20:32,482,216–32,484,943 | 275.8 kb | Distal (>10kb) Multiome | 976 |
Genomic view of the POFUT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.