Transcription factors with Perturb-seq knockdown data for PIWIL4-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PIWIL4-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PIWIL4-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:94,493,226–94,494,559 | 246.5 kb | Distal (>10kb) Multiome | 772 | |
| chr11:94,512,016–94,512,839 | 227.9 kb | Distal (>10kb) Multiome | 343 | |
| chr11:94,543,659–94,545,776 | 196.1 kb | Distal (>10kb) Multiome | 824 | |
| chr11:94,643,177–94,643,647 | 96.9 kb | Distal (>10kb) Multiome HiCAR | 75 | |
| chr11:94,649,720–94,650,679 | 90.2 kb | Distal (>10kb) Multiome HiCAR | 129 | |
| chr11:94,734,354–94,735,444 | 5.7 kb | Proximal (<10kb) Multiome | 313 | |
| chr11:94,740,333–94,741,667 | 353 bp | At TSS Multiome | 433 | |
| chr11:94,746,507–94,747,457 | 6.2 kb | Proximal (<10kb) | 189 | |
| chr11:94,766,900–94,769,104 | 26.8 kb | Distal (>10kb) Multiome | 701 | |
| chr11:94,866,030–94,867,452 | 126.3 kb | Distal (>10kb) Multiome | 112 | |
| chr11:94,973,096–94,974,006 | 233.3 kb | Distal (>10kb) Multiome | 740 |
Genomic view of the PIWIL4-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.