This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PIAS3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PIAS3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PIAS3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:145,607,803–145,608,364 | 251.8 kb | Distal (>10kb) Multiome | 205 | |
| chr1:145,707,296–145,708,738 | 151.6 kb | Distal (>10kb) Multiome | 511 | |
| chr1:145,720,881–145,721,650 | 138.5 kb | Distal (>10kb) Multiome | 548 | |
| chr1:145,823,605–145,824,539 | 35.7 kb | Distal (>10kb) Multiome | 899 | |
| chr1:145,845,315–145,846,128 | 14.0 kb | Distal (>10kb) Multiome | 750 | |
| chr1:145,858,348–145,860,193 | 208 bp | At TSS Multiome | 828 | |
| chr1:145,885,653–145,886,460 | 26.3 kb | Distal (>10kb) Multiome | 327 | |
| chr1:145,891,718–145,892,684 | 32.3 kb | Distal (>10kb) Multiome | 738 | |
| chr1:145,918,087–145,919,947 | 59.1 kb | Distal (>10kb) Multiome | 724 | |
| chr1:145,926,848–145,927,896 | 67.7 kb | Distal (>10kb) Multiome | 890 | |
| chr1:145,932,283–145,933,321 | 73.0 kb | Distal (>10kb) Multiome | 308 | |
| chr1:145,957,545–145,958,280 | 98.2 kb | Distal (>10kb) Multiome | 631 | |
| chr1:145,960,287–145,961,311 | 100.9 kb | Distal (>10kb) Multiome | 498 | |
| chr1:145,964,302–145,964,971 | 104.9 kb | Distal (>10kb) Multiome | 662 | |
| chr1:145,978,977–145,980,010 | 119.8 kb | Distal (>10kb) Multiome | 689 | |
| chr1:145,996,493–145,997,058 | 136.9 kb | Distal (>10kb) Multiome | 911 | |
| chr1:146,035,364–146,036,312 | 175.9 kb | Distal (>10kb) Multiome | 845 | |
| chr1:146,036,931–146,039,723 | 177.3 kb | Distal (>10kb) Multiome | 1048 | |
| chr1:146,051,092–146,052,696 | 192.5 kb | Distal (>10kb) Multiome | 1182 |
Genomic view of the PIAS3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.