PEMT

phosphatidylethanolamine N-methyltransferase | PEMPT, PEMT2

Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]

Biological processes 16 terms

S-adenosylmethionine-dependent methyltransferase activity (GO:0008757)endoplasmic reticulum (GO:0005783)endoplasmic reticulum (GO:0005783)endoplasmic reticulum membrane (GO:0005789)endoplasmic reticulum membrane (GO:0005789)mitochondrial membrane (GO:0031966)phosphatidyl-N-methylethanolamine N-methyltransferase activity (GO:0000773)phosphatidylcholine biosynthetic process (GO:0006656)phosphatidylcholine biosynthetic process (GO:0006656)phosphatidylcholine biosynthetic process (GO:0006656)phosphatidylethanolamine N-methyltransferase activity (GO:0004608)phosphatidylethanolamine N-methyltransferase activity (GO:0004608)phosphatidylethanolamine N-methyltransferase activity (GO:0004608)positive regulation of cold-induced thermogenesis (GO:0120162)positive regulation of cold-induced thermogenesis (GO:0120162)protein binding (GO:0005515)

Expression (TPM)

TFs regulating PEMT 0 TFs

Transcription factors with Perturb-seq knockdown data for PEMT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PEMT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to PEMT

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PEMT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr17:17,300,712–17,301,575	290.5 kb	Distal (>10kb) Multiome	301
chr17:17,303,177–17,303,990	288.2 kb	Distal (>10kb) Multiome	535
chr17:17,476,268–17,477,427	114.7 kb	Distal (>10kb) Multiome	720
chr17:17,494,918–17,497,329	95.2 kb	Distal (>10kb) Multiome	704
chr17:17,591,221–17,592,219	45 bp	At TSS Multiome	945
chr17:17,680,744–17,682,037	89.6 kb	Distal (>10kb) Multiome	507
chr17:17,682,791–17,683,426	91.3 kb	Distal (>10kb) Multiome	510
chr17:17,693,050–17,694,108	101.7 kb	Distal (>10kb) Multiome	549
chr17:17,694,284–17,695,355	103.1 kb	Distal (>10kb) Multiome	703
chr17:17,700,114–17,700,885	108.8 kb	Distal (>10kb) Multiome	327
chr17:17,724,458–17,725,494	133.4 kb	Distal (>10kb) Multiome	283
chr17:17,751,962–17,752,874	160.6 kb	Distal (>10kb) Multiome	640
chr17:17,780,889–17,783,985	189.4 kb	Distal (>10kb) Multiome	655
chr17:17,792,432–17,793,087	201.2 kb	Distal (>10kb) Multiome	135
chr17:17,809,458–17,811,066	218.3 kb	Distal (>10kb) Multiome	371
chr17:17,811,471–17,812,921	220.7 kb	Distal (>10kb) Multiome	664
chr17:17,823,156–17,824,542	232.1 kb	Distal (>10kb) Multiome	743
chr17:17,835,765–17,838,695	244.7 kb	Distal (>10kb) Multiome	1030
chr17:17,839,788–17,841,046	248.6 kb	Distal (>10kb) Multiome	927

Genome Browser

Genomic view of the PEMT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.