P4HA3

prolyl 4-hydroxylase subunit alpha 3 | C-P4Halpha(III)

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Biological processes 10 terms

L-ascorbic acid binding (GO:0031418)endoplasmic reticulum (GO:0005783)endoplasmic reticulum lumen (GO:0005788)endoplasmic reticulum lumen (GO:0005788)iron ion binding (GO:0005506)oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen (GO:0016705)procollagen-proline 4-dioxygenase activity (GO:0004656)procollagen-proline 4-dioxygenase activity (GO:0004656)procollagen-proline 4-dioxygenase complex (GO:0016222)protein binding (GO:0005515)

Expression (TPM)

TFs regulating P4HA3 0 TFs

Transcription factors with Perturb-seq knockdown data for P4HA3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = P4HA3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to P4HA3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of P4HA3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr11:74,170,498–74,171,610	140.5 kb	Distal (>10kb) Multiome	881
chr11:74,311,252–74,311,993	at TSS	At TSS Multiome	289
chr11:74,397,796–74,399,222	87.0 kb	Distal (>10kb) Multiome	811
chr11:74,467,393–74,467,865	155.8 kb	Distal (>10kb) Multiome	174
chr11:74,492,667–74,494,411	181.8 kb	Distal (>10kb) Multiome	897
chr11:74,592,212–74,593,000	280.9 kb	Distal (>10kb) Multiome	716

Genome Browser

Genomic view of the P4HA3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.