Transcription factors with Perturb-seq knockdown data for NKX2-1-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NKX2-1-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NKX2-1-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:36,510,281–36,510,748 | 8.5 kb | Proximal (<10kb) | 44 | |
| chr14:36,512,403–36,513,922 | 5.4 kb | Proximal (<10kb) | 230 | |
| chr14:36,515,024–36,515,174 | 4.1 kb | Proximal (<10kb) | 37 | |
| chr14:36,517,184–36,521,366 | at TSS | At TSS | 575 | |
| chr14:36,521,722–36,522,009 | 2.4 kb | Proximal (<10kb) | 72 | |
| chr14:36,522,279–36,522,938 | 3.0 kb | Proximal (<10kb) | 65 | |
| chr14:36,524,258–36,524,418 | 5.0 kb | Proximal (<10kb) | 53 | |
| chr14:36,524,671–36,525,867 | 5.4 kb | Proximal (<10kb) | 355 |
Genomic view of the NKX2-1-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.