Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]
Transcription factors with Perturb-seq knockdown data for NFS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NFS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NFS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:35,454,521–35,455,845 | 244.1 kb | Distal (>10kb) Multiome | 1059 | |
| chr20:35,539,672–35,540,321 | 159.3 kb | Distal (>10kb) Multiome | 311 | |
| chr20:35,541,280–35,542,685 | 157.2 kb | Distal (>10kb) Multiome | 849 | |
| chr20:35,600,739–35,601,999 | 97.7 kb | Distal (>10kb) Multiome | 575 | |
| chr20:35,604,921–35,605,589 | 94.0 kb | Distal (>10kb) Multiome | 27 | |
| chr20:35,615,559–35,617,919 | 81.5 kb | Distal (>10kb) Multiome | 1169 | |
| chr20:35,618,589–35,619,849 | 79.8 kb | Distal (>10kb) Multiome | 865 | |
| chr20:35,664,428–35,665,171 | 34.4 kb | Distal (>10kb) Multiome | 1018 | |
| chr20:35,698,724–35,699,893 | 72 bp | At TSS Multiome | 1035 | |
| chr20:35,741,564–35,742,903 | 43.1 kb | Distal (>10kb) Multiome | 1145 | |
| chr20:35,769,101–35,769,560 | 70.0 kb | Distal (>10kb) Multiome | 415 | |
| chr20:35,771,622–35,772,463 | 72.8 kb | Distal (>10kb) Multiome | 998 | |
| chr20:35,953,663–35,955,230 | 255.3 kb | Distal (>10kb) Multiome | 1166 |
Genomic view of the NFS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.