Enables WW domain binding activity; identical protein binding activity; and sequence-specific double-stranded DNA binding activity. Contributes to cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including labyrinthine layer blood vessel development; negative regulation of bone mineralization; and positive regulation of peptidyl-lysine acetylation. Part of RNA polymerase II transcription regulator complex and protein-DNA complex. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for NFE2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NFE2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NFE2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:54,294,279–54,294,500 | 6.5 kb | Proximal (<10kb) | 534 | |
| chr12:54,295,763–54,296,162 | 4.9 kb | Proximal (<10kb) | 363 | |
| chr12:54,300,732–54,301,299 | at TSS | At TSS | 545 | |
| chr12:54,301,423–54,301,627 | 408 bp | At TSS | 278 |
Genomic view of the NFE2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.