Transcription factors with Perturb-seq knockdown data for NEMP2-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NEMP2-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NEMP2-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:190,319,052–190,320,177 | 215.0 kb | Distal (>10kb) Multiome | 934 | |
| chr2:190,343,052–190,344,699 | 190.9 kb | Distal (>10kb) Multiome | 922 | |
| chr2:190,407,333–190,409,184 | 126.5 kb | Distal (>10kb) Multiome | 669 | |
| chr2:190,526,525–190,526,722 | 8.1 kb | Proximal (<10kb) | 26 | |
| chr2:190,527,646–190,527,855 | 7.0 kb | Proximal (<10kb) | 12 | |
| chr2:190,533,850–190,535,346 | 190 bp | At TSS Multiome | 1040 | |
| chr2:190,647,806–190,650,037 | 114.2 kb | Distal (>10kb) Multiome | 864 | |
| chr2:190,679,871–190,680,604 | 145.5 kb | Distal (>10kb) Multiome | 345 |
Genomic view of the NEMP2-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.