The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NELFCD. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NELFCD upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NELFCD, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:58,692,413–58,693,477 | 288.5 kb | Distal (>10kb) Multiome | 317 | |
| chr20:58,850,471–58,851,960 | 130.3 kb | Distal (>10kb) Multiome | 308 | |
| chr20:58,852,406–58,852,990 | 128.5 kb | Distal (>10kb) Multiome | 172 | |
| chr20:58,888,196–58,892,842 | 92.2 kb | Distal (>10kb) Multiome | 977 | |
| chr20:58,980,774–58,981,785 | 4 bp | At TSS Multiome | 797 | |
| chr20:58,981,896–58,982,243 | 622 bp | At TSS | 294 | |
| chr20:59,006,785–59,007,608 | 26.0 kb | Distal (>10kb) Multiome | 401 | |
| chr20:59,031,825–59,033,029 | 51.2 kb | Distal (>10kb) Multiome | 1108 | |
| chr20:59,042,196–59,043,527 | 61.6 kb | Distal (>10kb) Multiome | 834 | |
| chr20:59,222,192–59,223,002 | 241.4 kb | Distal (>10kb) Multiome | 395 | |
| chr20:59,403,591–59,404,046 | 422.6 kb | Distal (>10kb) Multiome HiCAR | 434 |
Genomic view of the NELFCD locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.