This gene encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified. [provided by RefSeq, Mar 2017]
Transcription factors with Perturb-seq knockdown data for NEIL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NEIL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NEIL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:11,563,746–11,564,943 | 205.0 kb | Distal (>10kb) Multiome HiCAR | 644 | |
| chr8:11,617,248–11,618,158 | 151.9 kb | Distal (>10kb) Multiome | 126 | |
| chr8:11,632,784–11,633,414 | 136.6 kb | Distal (>10kb) Multiome | 233 | |
| chr8:11,642,077–11,643,101 | 127.1 kb | Distal (>10kb) Multiome | 247 | |
| chr8:11,660,832–11,661,535 | 108.5 kb | Distal (>10kb) Multiome | 21 | |
| chr8:11,682,733–11,683,887 | 86.5 kb | Distal (>10kb) Multiome | 157 | |
| chr8:11,702,714–11,704,461 | 66.6 kb | Distal (>10kb) Multiome | 687 | |
| chr8:11,708,271–11,710,646 | 59.6 kb | Distal (>10kb) Multiome | 246 | |
| chr8:11,736,329–11,736,895 | 33.2 kb | Distal (>10kb) Multiome | 615 | |
| chr8:11,743,316–11,744,611 | 25.4 kb | Distal (>10kb) Multiome | 243 | |
| chr8:11,765,293–11,765,751 | 3.9 kb | Proximal (<10kb) | 302 | |
| chr8:11,769,172–11,770,442 | 2 bp | At TSS Multiome | 764 | |
| chr8:11,801,766–11,803,423 | 32.9 kb | Distal (>10kb) Multiome | 901 | |
| chr8:11,807,986–11,809,046 | 38.7 kb | Distal (>10kb) Multiome | 514 | |
| chr8:11,867,298–11,868,997 | 98.4 kb | Distal (>10kb) Multiome | 752 | |
| chr8:11,902,256–11,902,767 | 132.8 kb | Distal (>10kb) Multiome HiCAR | 604 | |
| chr8:11,986,166–11,987,022 | 216.9 kb | Distal (>10kb) Multiome | 233 |
Genomic view of the NEIL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.