Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NARF. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NARF upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NARF, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:82,212,405–82,213,592 | 245.8 kb | Distal (>10kb) Multiome | 911 | |
| chr17:82,228,266–82,232,536 | 229.7 kb | Distal (>10kb) Multiome | 1048 | |
| chr17:82,272,608–82,274,062 | 185.0 kb | Distal (>10kb) Multiome | 943 | |
| chr17:82,278,488–82,279,226 | 179.8 kb | Distal (>10kb) Multiome | 229 | |
| chr17:82,292,400–82,293,256 | 165.8 kb | Distal (>10kb) Multiome | 944 | |
| chr17:82,414,581–82,415,044 | 43.8 kb | Distal (>10kb) Multiome | 799 | |
| chr17:82,417,993–82,419,018 | 40.2 kb | Distal (>10kb) Multiome | 844 | |
| chr17:82,450,369–82,451,002 | 7.9 kb | Proximal (<10kb) Multiome | 837 | |
| chr17:82,458,067–82,459,318 | 136 bp | At TSS Multiome | 1054 | |
| chr17:82,494,122–82,495,128 | 36.0 kb | Distal (>10kb) Multiome | 1010 | |
| chr17:82,496,968–82,497,781 | 38.6 kb | Distal (>10kb) Multiome | 613 | |
| chr17:82,519,154–82,520,226 | 60.9 kb | Distal (>10kb) Multiome | 869 | |
| chr17:82,647,682–82,648,684 | 189.9 kb | Distal (>10kb) Multiome | 874 | |
| chr17:82,697,983–82,699,271 | 240.1 kb | Distal (>10kb) Multiome | 623 | |
| chr17:82,715,983–82,717,178 | 257.9 kb | Distal (>10kb) Multiome | 744 | |
| chr17:82,735,174–82,736,034 | 276.8 kb | Distal (>10kb) Multiome | 649 | |
| chr17:82,751,094–82,752,780 | 293.1 kb | Distal (>10kb) Multiome | 987 | |
| chr17:82,754,710–82,755,244 | 296.2 kb | Distal (>10kb) Multiome | 188 |
Genomic view of the NARF locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.