This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NAB2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NAB2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NAB2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:57,006,056–57,006,942 | 82.6 kb | Distal (>10kb) Multiome | 422 | |
| chr12:57,078,210–57,079,177 | 10.3 kb | Distal (>10kb) Multiome | 778 | |
| chr12:57,086,367–57,089,469 | 2.6 kb | Proximal (<10kb) Multiome | 1047 | |
| chr12:57,089,684–57,090,257 | 571 bp | At TSS | 405 | |
| chr12:57,093,335–57,093,558 | 4.2 kb | Proximal (<10kb) | 247 | |
| chr12:57,093,695–57,094,140 | 5.0 kb | Proximal (<10kb) Multiome | 243 | |
| chr12:57,110,045–57,111,598 | 21.3 kb | Distal (>10kb) Multiome | 656 | |
| chr12:57,111,611–57,112,633 | 22.8 kb | Distal (>10kb) Multiome | 940 | |
| chr12:57,127,277–57,129,227 | 39.3 kb | Distal (>10kb) Multiome | 725 | |
| chr12:57,215,826–57,217,972 | 127.5 kb | Distal (>10kb) Multiome | 525 | |
| chr12:57,224,526–57,225,152 | 135.9 kb | Distal (>10kb) Multiome | 267 | |
| chr12:57,225,987–57,227,617 | 138.0 kb | Distal (>10kb) Multiome | 236 | |
| chr12:57,229,358–57,230,407 | 140.8 kb | Distal (>10kb) Multiome | 739 | |
| chr12:57,237,805–57,241,749 | 150.1 kb | Distal (>10kb) Multiome | 846 | |
| chr12:57,242,371–57,244,402 | 154.6 kb | Distal (>10kb) Multiome | 596 |
Genomic view of the NAB2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.