This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Mar 2023]
Transcription factors with Perturb-seq knockdown data for N6AMT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = N6AMT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of N6AMT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr21:28,884,634–28,884,761 | 605 bp | At TSS | 96 | |
| chr21:28,884,897–28,885,668 | 110 bp | At TSS Multiome | 1089 | |
| chr21:28,922,250–28,923,423 | 37.2 kb | Distal (>10kb) Multiome | 112 | |
| chr21:28,992,126–28,993,365 | 107.6 kb | Distal (>10kb) Multiome | 927 | |
| chr21:29,002,426–29,003,155 | 117.4 kb | Distal (>10kb) Multiome | 924 | |
| chr21:29,018,921–29,019,505 | 133.9 kb | Distal (>10kb) Multiome | 771 | |
| chr21:29,024,275–29,025,379 | 139.4 kb | Distal (>10kb) Multiome | 850 | |
| chr21:29,072,977–29,074,186 | 188.4 kb | Distal (>10kb) Multiome | 1002 |
Genomic view of the N6AMT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.