Transcription factors with Perturb-seq knockdown data for MYOSLID. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MYOSLID upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MYOSLID, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:206,954,806–206,955,846 | 210.8 kb | Distal (>10kb) Multiome | 169 | |
| chr2:207,122,810–207,124,383 | 42.4 kb | Distal (>10kb) Multiome | 254 | |
| chr2:207,148,801–207,149,994 | 16.7 kb | Distal (>10kb) Multiome | 202 | |
| chr2:207,155,781–207,156,334 | 9.8 kb | Proximal (<10kb) | 36 | |
| chr2:207,163,642–207,163,838 | 2.3 kb | Proximal (<10kb) | 214 | |
| chr2:207,164,974–207,167,487 | 172 bp | At TSS Multiome | 862 | |
| chr2:207,201,001–207,201,673 | 35.3 kb | Distal (>10kb) Multiome | 71 | |
| chr2:207,202,647–207,203,423 | 36.9 kb | Distal (>10kb) Multiome | 138 | |
| chr2:207,226,642–207,227,725 | 61.1 kb | Distal (>10kb) Multiome | 248 | |
| chr2:207,408,155–207,408,852 | 242.4 kb | Distal (>10kb) Multiome | 224 | |
| chr2:207,473,001–207,473,846 | 307.4 kb | Distal (>10kb) Multiome HiCAR | 203 |
Genomic view of the MYOSLID locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.