Transcription factors with Perturb-seq knockdown data for MISP3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MISP3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MISP3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:14,071,936–14,072,417 | 1.2 kb | Proximal (<10kb) | 676 | |
| chr19:14,072,541–14,072,834 | 746 bp | At TSS | 384 | |
| chr19:14,072,991–14,073,911 | at TSS | At TSS | 589 | |
| chr19:14,075,132–14,075,358 | 1.6 kb | Proximal (<10kb) | 570 | |
| chr19:14,081,022–14,082,024 | 7.4 kb | Proximal (<10kb) | 806 |
Genomic view of the MISP3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.