There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MGAT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MGAT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MGAT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:180,618,726–180,619,463 | 191.0 kb | Distal (>10kb) Multiome | 289 | |
| chr5:180,648,868–180,649,861 | 160.9 kb | Distal (>10kb) Multiome | 281 | |
| chr5:180,802,126–180,804,080 | 7.2 kb | Proximal (<10kb) Multiome | 796 | |
| chr5:180,804,100–180,804,921 | 1.3 kb | Proximal (<10kb) | 215 | |
| chr5:180,805,012–180,805,347 | 2.2 kb | Proximal (<10kb) | 108 | |
| chr5:180,808,516–180,808,935 | 5.7 kb | Proximal (<10kb) | 204 | |
| chr5:180,809,580–180,811,300 | 179 bp | At TSS Multiome | 1018 | |
| chr5:180,831,123–180,831,970 | 21.4 kb | Distal (>10kb) Multiome | 549 | |
| chr5:180,860,430–180,861,900 | 50.5 kb | Distal (>10kb) Multiome HiCAR | 974 | |
| chr5:181,027,794–181,028,639 | 218.1 kb | Distal (>10kb) Multiome | 337 | |
| chr5:181,069,860–181,070,513 | 260.1 kb | Distal (>10kb) Multiome | 136 |
Genomic view of the MGAT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.