Transcription factors with Perturb-seq knockdown data for METTL26. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = METTL26 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of METTL26, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:351,804–354,436 | 283.4 kb | Distal (>10kb) Multiome | 654 | |
| chr16:378,999–379,813 | 257.0 kb | Distal (>10kb) Multiome | 225 | |
| chr16:380,836–382,614 | 254.4 kb | Distal (>10kb) Multiome | 634 | |
| chr16:383,438–384,218 | 252.5 kb | Distal (>10kb) Multiome | 342 | |
| chr16:396,893–397,640 | 238.8 kb | Distal (>10kb) Multiome | 496 | |
| chr16:401,380–402,199 | 234.5 kb | Distal (>10kb) Multiome | 818 | |
| chr16:424,888–426,136 | 210.7 kb | Distal (>10kb) Multiome | 752 | |
| chr16:483,333–484,000 | 152.6 kb | Distal (>10kb) Multiome | 143 | |
| chr16:526,749–528,601 | 108.7 kb | Distal (>10kb) Multiome | 811 | |
| chr16:569,530–570,338 | 66.5 kb | Distal (>10kb) Multiome | 492 | |
| chr16:578,896–579,353 | 57.2 kb | Distal (>10kb) Multiome | 511 | |
| chr16:588,593–590,724 | 46.5 kb | Distal (>10kb) Multiome | 853 | |
| chr16:630,800–631,439 | 4.9 kb | Proximal (<10kb) | 392 | |
| chr16:635,900–638,127 | 25 bp | At TSS Multiome | 1021 | |
| chr16:641,491–643,007 | 5.5 kb | Proximal (<10kb) Multiome | 856 | |
| chr16:648,795–649,829 | 13.0 kb | Distal (>10kb) Multiome | 862 | |
| chr16:660,457–661,656 | 24.6 kb | Distal (>10kb) Multiome | 456 | |
| chr16:667,633–668,442 | 31.7 kb | Distal (>10kb) Multiome | 838 | |
| chr16:678,847–681,585 | 43.7 kb | Distal (>10kb) Multiome | 803 | |
| chr16:683,618–684,859 | 48.1 kb | Distal (>10kb) Multiome | 701 | |
| chr16:694,897–695,485 | 59.1 kb | Distal (>10kb) Multiome | 390 | |
| chr16:705,242–706,468 | 69.6 kb | Distal (>10kb) Multiome | 248 | |
| chr16:714,577–716,048 | 78.8 kb | Distal (>10kb) Multiome | 555 | |
| chr16:720,434–721,963 | 85.0 kb | Distal (>10kb) Multiome | 966 | |
| chr16:726,310–729,144 | 90.7 kb | Distal (>10kb) Multiome | 736 | |
| chr16:740,359–741,596 | 104.7 kb | Distal (>10kb) Multiome | 897 | |
| chr16:787,564–789,160 | 152.2 kb | Distal (>10kb) Multiome | 635 | |
| chr16:807,352–808,044 | 171.5 kb | Distal (>10kb) Multiome | 600 | |
| chr16:813,330–813,960 | 177.4 kb | Distal (>10kb) Multiome | 325 |
Genomic view of the METTL26 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.