This gene encodes a non-protein coding antisense RNA that is imprinted, and preferentially expressed from the paternal allele. It is located in an intron of MEST gene in the opposite orientation, and shares the promoter region in common with one of the promoters (P2) of MEST gene. The antisense RNA is expressed predominantly in testis and mature motile spermatozoa, suggesting that it may be involved in the regulation of MEST expression during development.[provided by RefSeq, Oct 2010]
Transcription factors with Perturb-seq knockdown data for MESTIT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MESTIT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MESTIT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:130,485,879–130,487,031 | 4.0 kb | Proximal (<10kb) | 696 | |
| chr7:130,490,902–130,491,116 | at TSS | At TSS | 108 | |
| chr7:130,491,258–130,491,648 | 225 bp | At TSS | 91 | |
| chr7:130,491,946–130,492,475 | 913 bp | At TSS | 133 |
Genomic view of the MESTIT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.