The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MED9. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED9 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED9, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:17,236,752–17,237,798 | 239.7 kb | Distal (>10kb) Multiome | 841 | |
| chr17:17,280,620–17,281,467 | 196.0 kb | Distal (>10kb) Multiome | 835 | |
| chr17:17,300,712–17,301,575 | 175.8 kb | Distal (>10kb) Multiome | 301 | |
| chr17:17,303,177–17,303,990 | 173.6 kb | Distal (>10kb) Multiome | 535 | |
| chr17:17,476,268–17,477,427 | 10 bp | At TSS Multiome | 720 | |
| chr17:17,494,918–17,497,329 | 19.5 kb | Distal (>10kb) Multiome | 704 | |
| chr17:17,591,221–17,592,219 | 114.7 kb | Distal (>10kb) Multiome | 945 | |
| chr17:17,680,744–17,682,037 | 204.3 kb | Distal (>10kb) Multiome | 507 | |
| chr17:17,682,791–17,683,426 | 206.0 kb | Distal (>10kb) Multiome | 510 | |
| chr17:17,693,050–17,694,108 | 216.4 kb | Distal (>10kb) Multiome | 549 | |
| chr17:17,694,284–17,695,355 | 217.8 kb | Distal (>10kb) Multiome | 703 | |
| chr17:17,700,114–17,700,885 | 223.5 kb | Distal (>10kb) Multiome | 327 | |
| chr17:17,724,458–17,725,494 | 248.1 kb | Distal (>10kb) Multiome | 283 | |
| chr17:17,751,962–17,752,874 | 275.3 kb | Distal (>10kb) Multiome | 640 |
Genomic view of the MED9 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.