MED8

mediator complex subunit 8 | ARC32, MGC17544, MGC19641

This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Member of: DE-1

Biological processes 17 terms

RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II preinitiation complex assembly (GO:0051123)core mediator complex (GO:0070847)mediator complex (GO:0016592)mediator complex (GO:0016592)mediator complex (GO:0016592)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of transcription elongation by RNA polymerase II (GO:0032968)positive regulation of transcription initiation by RNA polymerase II (GO:0060261)protein binding (GO:0005515)protein ubiquitination (GO:0016567)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)transcription coregulator activity (GO:0003712)transcription coregulator activity (GO:0003712)

Expression (TPM)

TFs regulating MED8 0 TFs

Transcription factors with Perturb-seq knockdown data for MED8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to MED8

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr1:43,147,490–43,148,180	242.0 kb	Distal (>10kb) Multiome	318
chr1:43,172,043–43,172,806	217.3 kb	Distal (>10kb) Multiome	767
chr1:43,270,344–43,271,458	119.0 kb	Distal (>10kb) Multiome HiCAR	301
chr1:43,285,284–43,285,996	104.1 kb	Distal (>10kb) Multiome	403
chr1:43,348,408–43,350,097	40.5 kb	Distal (>10kb) Multiome	819
chr1:43,358,112–43,359,560	31.0 kb	Distal (>10kb) Multiome	818
chr1:43,366,277–43,366,800	23.3 kb	Distal (>10kb) Multiome	226
chr1:43,366,988–43,368,619	21.6 kb	Distal (>10kb) Multiome	991
chr1:43,389,284–43,390,213	34 bp	At TSS Multiome	905
chr1:43,452,851–43,454,257	63.6 kb	Distal (>10kb) Multiome	455
chr1:43,454,433–43,454,997	64.9 kb	Distal (>10kb) Multiome	267
chr1:43,530,512–43,532,007	141.2 kb	Distal (>10kb) Multiome	520
chr1:43,545,657–43,546,353	156.2 kb	Distal (>10kb) Multiome	445
chr1:43,549,934–43,550,659	160.5 kb	Distal (>10kb) Multiome	343
chr1:43,565,502–43,566,158	176.0 kb	Distal (>10kb) Multiome	173
chr1:43,649,463–43,650,795	260.3 kb	Distal (>10kb) Multiome	997

Genome Browser

Genomic view of the MED8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.