MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008]
Transcription factors with Perturb-seq knockdown data for MED10. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED10 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED10, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:6,377,871–6,379,073 | 61 bp | At TSS Multiome | 776 | |
| chr5:6,381,030–6,381,446 | 2.5 kb | Proximal (<10kb) | 4 | |
| chr5:6,475,554–6,476,306 | 97.3 kb | Distal (>10kb) Multiome | 93 | |
| chr5:6,582,577–6,583,754 | 204.7 kb | Distal (>10kb) Multiome | 244 | |
| chr5:6,595,290–6,596,038 | 217.1 kb | Distal (>10kb) Multiome | 157 | |
| chr5:6,632,465–6,634,316 | 254.7 kb | Distal (>10kb) Multiome | 837 |
Genomic view of the MED10 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.