This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Transcription factors with Perturb-seq knockdown data for MDFIC. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MDFIC upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MDFIC, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:114,918,399–114,918,997 | 3.6 kb | Proximal (<10kb) | 29 | |
| chr7:114,921,636–114,923,568 | 219 bp | At TSS Multiome | 624 | |
| chr7:114,930,063–114,930,569 | 7.5 kb | Proximal (<10kb) | 165 | |
| chr7:115,005,700–115,006,426 | 83.6 kb | Distal (>10kb) Multiome | 176 | |
| chr7:115,052,541–115,053,253 | 130.4 kb | Distal (>10kb) Multiome | 95 | |
| chr7:115,152,358–115,153,414 | 230.1 kb | Distal (>10kb) Multiome | 144 |
Genomic view of the MDFIC locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.