This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Transcription factors with Perturb-seq knockdown data for MCF2L. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCF2L upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCF2L, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr13:112,606,168–112,606,859 | 287.9 kb | Distal (>10kb) Multiome | 517 | |
| chr13:112,689,000–112,690,944 | 204.3 kb | Distal (>10kb) Multiome HiCAR | 778 | |
| chr13:112,711,516–112,712,479 | 182.5 kb | Distal (>10kb) Multiome | 761 | |
| chr13:112,885,816–112,886,312 | 8.4 kb | Proximal (<10kb) Multiome | 440 | |
| chr13:112,892,653–112,893,167 | 1.2 kb | Proximal (<10kb) | 292 | |
| chr13:112,893,373–112,895,600 | 149 bp | At TSS Multiome | 634 | |
| chr13:112,906,009–112,906,659 | 12.0 kb | Distal (>10kb) Multiome | 327 | |
| chr13:112,942,743–112,944,278 | 48.5 kb | Distal (>10kb) Multiome | 632 | |
| chr13:112,968,613–112,969,532 | 74.8 kb | Distal (>10kb) Multiome | 497 | |
| chr13:113,022,696–113,023,521 | 128.7 kb | Distal (>10kb) Multiome | 461 | |
| chr13:113,152,885–113,154,060 | 258.9 kb | Distal (>10kb) Multiome | 714 | |
| chr13:113,179,328–113,180,131 | 285.3 kb | Distal (>10kb) Multiome | 164 |
Genomic view of the MCF2L locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.