This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
Transcription factors with Perturb-seq knockdown data for MAPKAPK3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAPKAPK3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAPKAPK3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:50,320,603–50,322,739 | 295.6 kb | Distal (>10kb) Multiome | 816 | |
| chr3:50,324,456–50,325,020 | 292.4 kb | Distal (>10kb) Multiome | 392 | |
| chr3:50,327,933–50,328,581 | 288.9 kb | Distal (>10kb) Multiome | 697 | |
| chr3:50,336,856–50,338,227 | 279.6 kb | Distal (>10kb) Multiome | 952 | |
| chr3:50,340,624–50,341,322 | 276.2 kb | Distal (>10kb) Multiome HiCAR | 345 | |
| chr3:50,345,443–50,346,042 | 271.4 kb | Distal (>10kb) Multiome HiCAR | 209 | |
| chr3:50,350,496–50,351,298 | 266.2 kb | Distal (>10kb) Multiome | 902 | |
| chr3:50,358,889–50,360,012 | 257.5 kb | Distal (>10kb) Multiome | 796 | |
| chr3:50,364,552–50,365,867 | 251.9 kb | Distal (>10kb) Multiome | 331 | |
| chr3:50,435,932–50,436,533 | 180.9 kb | Distal (>10kb) Multiome | 248 | |
| chr3:50,502,505–50,504,160 | 113.5 kb | Distal (>10kb) Multiome | 312 | |
| chr3:50,567,311–50,568,123 | 49.4 kb | Distal (>10kb) Multiome | 376 | |
| chr3:50,568,824–50,569,934 | 47.8 kb | Distal (>10kb) Multiome | 901 | |
| chr3:50,611,315–50,612,530 | 5.2 kb | Proximal (<10kb) Multiome | 904 | |
| chr3:50,614,162–50,614,540 | 2.6 kb | Proximal (<10kb) | 211 | |
| chr3:50,615,997–50,617,867 | 26 bp | At TSS Multiome | 754 | |
| chr3:50,674,463–50,675,603 | 58.0 kb | Distal (>10kb) Multiome | 344 | |
| chr3:51,384,278–51,385,586 | 768.0 kb | Distal (>10kb) Multiome HiCAR | 787 | |
| chr3:51,390,699–51,392,749 | 774.0 kb | Distal (>10kb) Multiome HiCAR | 824 | |
| chr3:51,499,159–51,500,640 | 882.9 kb | Distal (>10kb) Multiome HiCAR | 838 | |
| chr3:51,538,310–51,539,294 | 921.6 kb | Distal (>10kb) Multiome HiCAR | 974 | |
| chr3:51,670,855–51,672,297 | 1054.1 kb | Distal (>10kb) Multiome HiCAR | 799 | |
| chr3:51,967,071–51,968,598 | 1350.4 kb | Distal (>10kb) Multiome HiCAR | 581 |
Genomic view of the MAPKAPK3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.