The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
Transcription factors with Perturb-seq knockdown data for LAMA3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LAMA3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LAMA3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:23,437,346–23,438,074 | 251.6 kb | Distal (>10kb) Multiome | 628 | |
| chr18:23,452,793–23,453,775 | 236.3 kb | Distal (>10kb) Multiome | 796 | |
| chr18:23,502,975–23,503,998 | 186.1 kb | Distal (>10kb) Multiome | 907 | |
| chr18:23,586,020–23,587,174 | 102.7 kb | Distal (>10kb) Multiome | 908 | |
| chr18:23,618,918–23,619,643 | 70.2 kb | Distal (>10kb) Multiome | 295 | |
| chr18:23,662,307–23,663,058 | 26.7 kb | Distal (>10kb) Multiome | 349 | |
| chr18:23,689,205–23,690,311 | 220 bp | At TSS Multiome | 469 |
Genomic view of the LAMA3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.