Transcription factors with Perturb-seq knockdown data for ITGB1-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ITGB1-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ITGB1-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:32,948,457–32,949,002 | 9.4 kb | Proximal (<10kb) | 314 | |
| chr10:32,950,586–32,951,108 | 7.3 kb | Proximal (<10kb) | 209 | |
| chr10:32,954,253–32,954,452 | 4.0 kb | Proximal (<10kb) | 81 | |
| chr10:32,956,436–32,956,727 | 1.7 kb | Proximal (<10kb) | 51 | |
| chr10:32,957,333–32,958,991 | 82 bp | At TSS Multiome | 981 | |
| chr10:32,960,217–32,960,369 | 1.8 kb | Proximal (<10kb) | 138 | |
| chr10:32,961,770–32,962,285 | 3.3 kb | Proximal (<10kb) | 240 | |
| chr10:32,971,511–32,972,247 | 8.6 kb | Proximal (<10kb) | 346 | |
| chr10:32,980,401–32,981,503 | 22.3 kb | Distal (>10kb) Multiome | 925 | |
| chr10:32,981,717–32,982,378 | 874 bp | At TSS | 243 | |
| chr10:32,982,499–32,982,939 | 1.7 kb | Proximal (<10kb) | 117 | |
| chr10:32,983,270–32,983,845 | 2.4 kb | Proximal (<10kb) | 374 | |
| chr10:33,005,307–33,006,128 | 47.3 kb | Distal (>10kb) Multiome | 624 |
Genomic view of the ITGB1-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.