Transcription factors with Perturb-seq knockdown data for IRS3P. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IRS3P upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IRS3P, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:100,563,960–100,564,138 | 6.0 kb | Proximal (<10kb) | 254 | |
| chr7:100,569,177–100,570,642 | at TSS | At TSS | 855 | |
| chr7:100,571,007–100,573,075 | 877 bp | At TSS | 388 | |
| chr7:100,573,670–100,574,027 | 3.5 kb | Proximal (<10kb) | 170 |
Genomic view of the IRS3P locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.