This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011]
Transcription factors with Perturb-seq knockdown data for IRAG1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IRAG1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IRAG1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:10,351,409–10,352,676 | 300.2 kb | Distal (>10kb) Multiome HiCAR | 677 | |
| chr11:10,450,400–10,451,424 | 201.1 kb | Distal (>10kb) Multiome | 419 | |
| chr11:10,455,858–10,456,376 | 196.1 kb | Distal (>10kb) Multiome | 394 | |
| chr11:10,540,488–10,541,619 | 111.0 kb | Distal (>10kb) Multiome | 859 | |
| chr11:10,654,354–10,654,559 | 2.2 kb | Proximal (<10kb) | 174 | |
| chr11:10,735,514–10,736,088 | 83.7 kb | Distal (>10kb) Multiome | 637 | |
| chr11:10,750,622–10,751,770 | 98.9 kb | Distal (>10kb) Multiome | 974 | |
| chr11:10,792,316–10,792,962 | 140.5 kb | Distal (>10kb) Multiome | 530 | |
| chr11:10,807,818–10,809,752 | 156.9 kb | Distal (>10kb) Multiome | 1038 | |
| chr11:10,857,279–10,858,931 | 205.9 kb | Distal (>10kb) Multiome | 958 | |
| chr11:10,930,673–10,932,142 | 279.2 kb | Distal (>10kb) Multiome | 512 | |
| chr11:10,933,806–10,934,381 | 281.9 kb | Distal (>10kb) Multiome | 447 |
Genomic view of the IRAG1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.