Transcription factors with Perturb-seq knockdown data for INSYN2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = INSYN2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of INSYN2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:169,790,646–169,791,734 | 189.4 kb | Distal (>10kb) Multiome HiCAR | 114 | |
| chr5:169,980,228–169,981,102 | 231 bp | At TSS Multiome | 296 | |
| chr5:169,992,697–169,993,853 | 12.8 kb | Distal (>10kb) Multiome HiCAR | 37 | |
| chr5:170,109,224–170,110,040 | 129.2 kb | Distal (>10kb) Multiome | 135 | |
| chr5:170,110,858–170,111,551 | 130.7 kb | Distal (>10kb) Multiome | 29 | |
| chr5:170,183,896–170,184,541 | 203.7 kb | Distal (>10kb) Multiome | 234 | |
| chr5:170,279,137–170,279,623 | 298.9 kb | Distal (>10kb) Multiome | 183 |
Genomic view of the INSYN2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.