Transcription factors with Perturb-seq knockdown data for ID2-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ID2-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ID2-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:8,676,120–8,676,376 | 2.5 kb | Proximal (<10kb) | 82 | |
| chr2:8,676,483–8,677,573 | 1.3 kb | Proximal (<10kb) | 319 | |
| chr2:8,677,675–8,679,558 | at TSS | At TSS | 902 | |
| chr2:8,680,181–8,680,695 | 1.3 kb | Proximal (<10kb) | 599 | |
| chr2:8,680,827–8,683,692 | 2.0 kb | Proximal (<10kb) | 1154 | |
| chr2:8,684,500–8,686,247 | 5.6 kb | Proximal (<10kb) | 951 |
Genomic view of the ID2-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.