Transcription factors with Perturb-seq knockdown data for ICMT-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ICMT-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ICMT-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:5,980,417–5,980,986 | 255.6 kb | Distal (>10kb) Multiome | 282 | |
| chr1:5,991,687–5,993,712 | 243.6 kb | Distal (>10kb) Multiome | 829 | |
| chr1:6,127,538–6,128,516 | 108.3 kb | Distal (>10kb) Multiome | 300 | |
| chr1:6,148,503–6,149,108 | 87.4 kb | Distal (>10kb) Multiome | 254 | |
| chr1:6,199,148–6,200,364 | 36.5 kb | Distal (>10kb) Multiome | 992 | |
| chr1:6,204,893–6,206,690 | 31.0 kb | Distal (>10kb) Multiome | 913 | |
| chr1:6,208,737–6,209,635 | 26.9 kb | Distal (>10kb) Multiome | 362 | |
| chr1:6,235,160–6,236,710 | 189 bp | At TSS Multiome | 673 | |
| chr1:6,238,731–6,239,096 | 2.5 kb | Proximal (<10kb) | 121 | |
| chr1:6,241,773–6,242,771 | 6.0 kb | Proximal (<10kb) Multiome | 241 | |
| chr1:6,244,532–6,247,144 | 9.7 kb | Proximal (<10kb) Multiome | 1073 | |
| chr1:6,248,779–6,250,153 | 13.3 kb | Distal (>10kb) Multiome | 287 | |
| chr1:6,259,688–6,261,954 | 23.9 kb | Distal (>10kb) Multiome | 550 | |
| chr1:6,392,724–6,394,325 | 157.3 kb | Distal (>10kb) Multiome | 819 | |
| chr1:6,418,998–6,420,339 | 183.5 kb | Distal (>10kb) Multiome | 335 | |
| chr1:6,440,165–6,441,114 | 204.4 kb | Distal (>10kb) Multiome | 215 | |
| chr1:6,446,981–6,449,427 | 211.7 kb | Distal (>10kb) Multiome | 665 | |
| chr1:6,455,053–6,455,773 | 219.1 kb | Distal (>10kb) Multiome | 105 | |
| chr1:6,470,716–6,471,189 | 234.7 kb | Distal (>10kb) Multiome | 148 | |
| chr1:6,471,308–6,471,972 | 235.4 kb | Distal (>10kb) Multiome | 192 | |
| chr1:6,486,194–6,486,953 | 250.4 kb | Distal (>10kb) Multiome | 416 | |
| chr1:6,489,772–6,491,050 | 254.3 kb | Distal (>10kb) Multiome | 676 | |
| chr1:6,497,162–6,497,841 | 261.3 kb | Distal (>10kb) Multiome | 324 |
Genomic view of the ICMT-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.