Transcription factors with Perturb-seq knockdown data for IBA57-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IBA57-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IBA57-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:228,162,701–228,162,919 | 2.6 kb | Proximal (<10kb) | 94 | |
| chr1:228,164,898–228,166,840 | at TSS | At TSS | 1011 | |
| chr1:228,173,677–228,174,151 | 8.2 kb | Proximal (<10kb) | 239 | |
| chr1:228,174,477–228,174,893 | 9.0 kb | Proximal (<10kb) | 254 |
Genomic view of the IBA57-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.