The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for HSBP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HSBP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HSBP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:83,807,528–83,808,459 | 41 bp | At TSS Multiome | 733 | |
| chr16:83,840,075–83,841,171 | 32.5 kb | Distal (>10kb) Multiome | 136 | |
| chr16:83,888,352–83,889,387 | 80.8 kb | Distal (>10kb) Multiome | 237 | |
| chr16:83,898,559–83,899,828 | 91.0 kb | Distal (>10kb) Multiome | 773 | |
| chr16:83,926,399–83,927,376 | 118.9 kb | Distal (>10kb) Multiome | 532 | |
| chr16:83,968,110–83,968,725 | 160.4 kb | Distal (>10kb) Multiome | 261 | |
| chr16:84,013,600–84,015,009 | 206.3 kb | Distal (>10kb) Multiome | 388 | |
| chr16:84,043,411–84,043,885 | 235.7 kb | Distal (>10kb) Multiome | 292 | |
| chr16:84,062,518–84,063,095 | 254.9 kb | Distal (>10kb) Multiome | 330 |
Genomic view of the HSBP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.