Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Transcription factors with Perturb-seq knockdown data for HRH2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HRH2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HRH2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:175,443,902–175,445,629 | 213.2 kb | Distal (>10kb) Multiome | 291 | |
| chr5:175,478,159–175,479,153 | 179.5 kb | Distal (>10kb) Multiome | 710 | |
| chr5:175,533,977–175,534,736 | 123.7 kb | Distal (>10kb) Multiome HiCAR | 438 | |
| chr5:175,657,660–175,658,971 | 32 bp | At TSS Multiome | 283 | |
| chr5:175,729,513–175,730,170 | 71.8 kb | Distal (>10kb) Multiome | 464 | |
| chr5:175,796,457–175,798,050 | 139.0 kb | Distal (>10kb) Multiome | 1084 | |
| chr5:175,871,487–175,872,415 | 213.7 kb | Distal (>10kb) Multiome | 832 |
Genomic view of the HRH2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.