Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
Transcription factors with Perturb-seq knockdown data for HMOX2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HMOX2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HMOX2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:4,183,149–4,184,737 | 292.4 kb | Distal (>10kb) Multiome | 944 | |
| chr16:4,244,460–4,244,980 | 231.8 kb | Distal (>10kb) Multiome | 290 | |
| chr16:4,253,312–4,254,436 | 222.4 kb | Distal (>10kb) Multiome | 960 | |
| chr16:4,263,483–4,264,402 | 212.6 kb | Distal (>10kb) Multiome | 258 | |
| chr16:4,271,070–4,274,312 | 202.4 kb | Distal (>10kb) Multiome | 1130 | |
| chr16:4,293,681–4,294,322 | 182.4 kb | Distal (>10kb) Multiome | 385 | |
| chr16:4,307,236–4,308,275 | 168.6 kb | Distal (>10kb) Multiome | 635 | |
| chr16:4,309,629–4,310,195 | 166.4 kb | Distal (>10kb) Multiome | 351 | |
| chr16:4,315,041–4,316,747 | 160.6 kb | Distal (>10kb) Multiome | 520 | |
| chr16:4,327,446–4,328,634 | 148.1 kb | Distal (>10kb) Multiome | 495 | |
| chr16:4,350,579–4,351,773 | 125.0 kb | Distal (>10kb) Multiome | 674 | |
| chr16:4,371,473–4,372,791 | 104.6 kb | Distal (>10kb) Multiome | 617 | |
| chr16:4,402,485–4,403,104 | 73.6 kb | Distal (>10kb) Multiome | 450 | |
| chr16:4,415,818–4,417,201 | 59.7 kb | Distal (>10kb) Multiome | 681 | |
| chr16:4,425,392–4,426,322 | 50.7 kb | Distal (>10kb) Multiome | 881 | |
| chr16:4,474,111–4,474,929 | 1.8 kb | Proximal (<10kb) Multiome | 626 | |
| chr16:4,476,027–4,477,237 | at TSS | At TSS Multiome | 841 | |
| chr16:4,537,639–4,538,998 | 62.3 kb | Distal (>10kb) Multiome | 1087 | |
| chr16:4,585,331–4,585,992 | 109.3 kb | Distal (>10kb) Multiome | 223 | |
| chr16:4,613,912–4,617,164 | 138.5 kb | Distal (>10kb) Multiome | 1079 | |
| chr16:4,624,301–4,625,703 | 148.3 kb | Distal (>10kb) Multiome | 866 | |
| chr16:4,693,016–4,694,663 | 217.2 kb | Distal (>10kb) Multiome | 730 | |
| chr16:4,733,836–4,735,221 | 258.0 kb | Distal (>10kb) Multiome | 858 | |
| chr16:4,766,728–4,767,628 | 290.9 kb | Distal (>10kb) Multiome | 795 | |
| chr16:4,768,438–4,769,162 | 292.4 kb | Distal (>10kb) Multiome | 677 |
Genomic view of the HMOX2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.